Fig. 2 | Signal Transduction and Targeted Therapy

Fig. 2

From: TRIM47 is a novel endothelial activation factor that aggravates lipopolysaccharide-induced acute lung injury in mice via K63-linked ubiquitination of TRAF2

Fig. 2

TRIM47 is highly expressed in vascular endothelial cells. a The relative expression of TRIM47 gene in different cells was detected by real-time PCR (n = 3). b Representative images of the immunoblots of TIRM47 and β-actin in different cells detected by western blot (up) and the relative quantitation of TIRM47 protein levels (down, n = 3). THP-1, human monocytic cell line, RAW264.7, murine macrophage cell line, HL-1, mouse cardiac muscle cell line, HUVEC, human umbilical vein endothelial cells, EA.hy926, human umbilical vein cell line, hCMEC/D3, human brain microvascular endothelial cell line, bEnd.3, mouse brain microvascular endothelial cell line, HEK293, Human embryonic kidney 293 cells, U251, human glioma cell line, HeLa, human cervical cancer cells, MDA-MB-231, human triple-negative breast cancer cell line, A549, human lung carcinoma cell line, and 3T3-L1, mouse preadipocytes. c Representative immunohistochemical images showing positive staining of TRIM47 in the vascular lining of multiple tissues (partial amplification from Fig. 1a, n = 3). Scale bar, 50 μm. The relative expression of 56 TRIM genes in (d) HUVECs and (e) hCMEC/D3 was detected by real-time PCR. ND: not detected (n = 3)

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