Fig. 7 | Signal Transduction and Targeted Therapy

Fig. 7

From: TRIM47 is a novel endothelial activation factor that aggravates lipopolysaccharide-induced acute lung injury in mice via K63-linked ubiquitination of TRAF2

Fig. 7

TRIM47 mediates K63-linked ubiquitylation and interacts with TRAF2. HUVECs were transfected with Flag or Flag-TRIM47 plasmid for 48 h and treated with TNFα for 15 min. Whole-cell lysates were immunoprecipitated with Flag antibody, and the precipitates were immunoblotted with (a) Ub, Ub-K48, Ub-K63, (b) TRAF2, TRAF6, TAK1, IKKγ, and IκBα antibodies (n = 3). HUVECs were treated with 10 ng/ml TNF-α for 0 and 15 min. Cell lysates were then immunoprecipitated using (c) TRIM47 and (d) TRAF2 antibodies. Representative images demonstrated the total, K48- and K63-linked ubiquitylation by western blot analysis using the indicated antibodies (n = 3). e The schematic diagram of the mechanism by which TRIM47 mediates TNFα-induced endothelial activation through promoting endothelial TRAF2-MAPK/NF-κB pro-inflammatory axis

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