Fig. 3

LRP1 is responsible for the endocytosis of IETP2. a–d Microscopy images showing the competing endocytosis of IETP2-Cy5.5 and Lix-FITC by HEI-OC1 cells. The endocytosis of IETP2-Cy5.5 was partially reduced by Lix-FITC. e-f Quantitative analysis of the fluorescence intensity in a–d. e Quantitative data showing the percentages of IETP2-Cy5.5-positive cells. f Quantitative data showing the mean intensity of IETP2-Cy5.5 in cells. g RT–PCR showing the knockdown (KD) efficiency of Lrp1 with two different gRNAs. h Western blot showing the expression of LRP1 after LRP1 knockdown with two different gRNAs. i, j Immunofluorescence images showing the expression of LRP1 in WT and LRP1-KD HEI-OC1 cells. k, l Microscopy images showing the endocytosis of IETP2 in WT or LRP1-KD HIE-OC1 cells. Greatly reduced endocytosis of IETP2 in LRP1-KD HEI-OC1 cells was observed. m Quantitative analysis of the images in k, l. n Flow cytometry showing the reduced endocytosis of IETP2 in LRP1-KD HEI-OC1 cells. Error bars represent the mean ± SD. N = 5 for e, f, m. N = 3 for g. *p < 0.05, ***p < 0.001. Scale bars: 20 µm