Fig. 5 | Signal Transduction and Targeted Therapy

Fig. 5

From: The heterogeneous immune landscape between lung adenocarcinoma and squamous carcinoma revealed by single-cell RNA sequencing

Fig. 5

Re-clustering and gene expression patterns of Mφ in LUAD and LUSC. a Clustering of 11,016 Mφs from all samples. Each dot corresponds to a single cell, colored according to cell type. b t-SNE of Mφ subtype in LUAD (top) and LUSC (bottom). c Violin plots of specific marker genes in each Mφ subcluster. d Box plot showing the differential expression of FABP4, FCN1, SPP1, and SELENOP in LUAD and LUSC. The P values by Wilcoxon tests are shown. e Differences in functional activities scored each Mφ subtype by ssGSEA based on the marker genes of M1, M2, angiogenesis and phagocytosis. f Heatmap of specific differentially expressed genes of each Mφ subtype and the GO annotation of these genes. g Heat map showing the different tumor-related molecules in various Mφ subtypes between LUAD and LUSC. h Multicolor IHC staining for verifying the high expression of antibody transcripts in Mφ from LUSC compared with LUAD. The scale bar represents 20 μm

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