Fig. 3

Gene transfection efficiency of TAT2-P1 in vivo. a The transfection efficiency of TAT-P1, TAT2-P1, and in vivo jetPEI in mouse lungs. The pCMV-Luc was packaged by the indicated vectors with the weight ratio (4:1) of peptide:DNA. The luciferase expression in mouse lungs was measured at 24 h post transfection. Luciferase expression in mouse lungs was normalized to TAT-P1 (1000). Mock means mice treated with 5% glucose without pCMV-Luc. * indicates P < 0.05 and ** indicates P < 0. b Hydrodynamic diameter of nanoparticles of plasmid DNA packaged by indicated vectors. Sizes were measured by DynaPro Plate Reader. Data were presented as mean ± SD of six independent experiments. c The representative pictures of transmission electron micrographs (TEM) of nanoparticles. Peptides (1 mg ml⁻¹ or 2 mg ml⁻¹ for TAT2-P1-2mg) were mixed with equal volume of plasmid DNA (0.25 mg ml⁻¹ or 0.5 mg ml⁻¹) for measuring hydrodynamic diameter or for TEM negative staining. Scale bars, 200 nm. d The expression of luciferase in mouse lungs transfected with nanoparticle TAT2-P1/pCMV-Luc (1 mg ml⁻¹/0.25 mg ml⁻¹) and TAT2-P1/pCMV-Luc (2 mg ml⁻¹/0.5 mg ml⁻¹), respectively. Data were presented as mean ± SD of more than four mice in each group. e Representative image of In Vivo Imaging System showed decreased luciferase expression of pCMV-Luc packaged by 2 mg ml⁻¹ of TAT2-P1 (TAT2-P1-2mg) in mouse lungs. The same amount of TAT2-P1/pCMV-Luc was inoculated to corresponding mouse lungs at 24 h before measuring luciferase expression. Mock, mouse lungs inoculated with TAT2-P1. * indicates P < 0.05. P values were calculated by the two-tailed Student’s t test