Fig. 3
From: SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis
CDK2 knockdown specifically impairs the activity of RdRp. a, b HEK293T cells expressing CoV-Gluc, nsp12, nsp7, nsp8 plasmid DNA at the ratio of 1:10:30:30 (a) or control vector and CoV-Gluc (b) were transfected with three siRNA specific sequence for CDK2. After 48 h, minus-strand or plus-strand Gluc-RNA was detected by qRT-PCR and protein expression was detected by Western blot analysis. c CoV-Gluc, nsp12, nsp7, nsp8 plasmid DNA were co-transfected in HEK293T cells at the ratio described above, and then a series of CDK2/CyclinA concentration plasmids were transfected into six-well plates. After 48 h, minus-strand Gluc-RNA was detected by qRT-PCR and protein expression was detected by western blot analysis. Data are presented as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 and ns not significant (two-tailed unpaired Student’s t-test)
