Fig. 1
From: Targeting PSAT1 to mitigate metastasis in tumors with p53-72Pro variant
PSAT1 is identified as a p5372P-interacting protein. a The migration of different tumor cell lines treated with vehicle or serine (500 μM) was detected by transwell assays. Scale bar, 100 μm. Data are means ± s.d. NS not significant, *P < 0.05, ***P < 0.001. b The graphic model of functional domains of p53 and the localization of the SNP (rs1042522). c, d The different variants of rs1042522 in p53. The frequency of SNP rs1042522 in different populations. The data were collected from 105516 samples from NCBI Allele Frequency Aggregator study (https://www.ncbi.nlm.nih.gov/snp/rs1042522). e Venn diagram showing the p53-72P-interacting protein, p53-72R-interacting protein, and the overlap of p53-interacting protein with the metabolism-associated protein. f Co-immunoprecipitation analysis of the interaction between endogenous PSAT1 and Flag-tagged p53-72P in HEK293T cells. g Co-immunoprecipitation analysis of the interaction between Flag-tagged PSAT1 and endogenous p53 in HEK293T cells. h Co-immunoprecipitation analysis of the interaction between Flag-tagged p53-72P/72R and endogenous PSAT1 in Hep3B cells. i Proximity ligation assay (PLA) in Hep3B cells transfected with vector, p53-72P and p53-72R for the interaction of p53 and PSAT1. Scale bar, 10 μm. Data are means ± s.d. ***P < 0.001. j Co-immunoprecipitation analysis of the interaction between Flag-tagged mutant p53 (72P, 72R, Y220C, R249S) and endogenous PSAT1 in HEK293T cells. k Co-immunoprecipitation analysis of the interaction between endogenous p53 and Flag-tagged wild type PSAT1 (WT) or mutant PSAT1 (R45A) in HEK293T cells. l Immunoblotting analysis of PSAT1 in tumor cell lines treated with or without serine supplementation (500 μM)
