Fig. 6
From: Targeting PSAT1 to mitigate metastasis in tumors with p53-72Pro variant
AOA impedes liver cancer growth and metastasis in vivo and in vitro. a Parental or PSAT1 knockout PLC/PRF/5 cells were tail intravenously injected into BALB/c nude mice and the mice were treated with vehicle or AOA (10 mg/kg/day). Scale bar, 100 μm. Lung metastases were then calculated after 14-day treatment. b Transwell assay showing the migration and invasion of Hep3B cells transfected with vector, p53-72P, and p53-72R, in combination with 20 μM AOA treatment. Scale bar, 100 μm. c Wound healing assay showing cell migration of parental or PSAT1 knockout PLC/PRF/5 cells after 48 h, in combination with or without 20 μM AOA treatment. Scale bar, 200 μm. d Transwell assay showing the migration and invasion of parental or PSAT1 knockout PLC/PRF/5 cells, in combination with or without 20 μM AOA treatment. Scale bar, 50 μm. e The inhibition rate in different cell lines treated with or without 10 μM AOA, in the presence or absence of regorafenib for 24 h. f The survival fraction of parental or PSAT1 knockout PLC/PRF/5 cells treated with or without 20 μM AOA. g The statistic cell viability of parental or PSAT1 knockout PLC/PRF/5 cells treated with or without 20 μM AOA. h–j Cell viability of Hep3B cells transfected with vector (h), p53-72P (i), and p53-72R (j) plasmids. Cells were treated with regorafenib alone or AOA (10 μM) plus regorafenib as indicated concentrations. k Left: representative images of isolated PDX tumor expressing p53-72P. Xenografts of mice in cohorts were treated with vehicle, AOA (10 mg/kg/day) or AOA plus regorafenib (10 mg/kg/day). Treatment was initiated 24 h after tumors reached 100 mm3. Scale bar, 5 mm. Right: the weight of individual tumors in left. l Left: representative images of isolated PDX tumor expressing p53-72R. Xenografts of mice in cohorts were treated with vehicle, AOA (10 mg/kg/day) or AOA plus regorafenib (10 mg/kg/day). Treatment was initiated 24 h after tumors reached 100 mm3. Scale bar, 5 mm. Right: the weight of individual tumors in left. Data are means ± s.d. NS not significant, **P < 0.01, ***P < 0.001
