Fig. 1 | Signal Transduction and Targeted Therapy

Fig. 1

From: Short-term tamoxifen administration improves hepatic steatosis and glucose intolerance through JNK/MAPK in mice

Fig. 1

Tamoxifen decreased hepatocyte lipotoxicity in vitro. a Huh7, HepG2, AML12 cells, and primary hepatocytes were seeded in six-well plates. After 12 h, 0.3 mM sodium palmitate was added to the medium and after 36 h, DMSO/tamoxifen (10, 20, 40 μM) was added. After 36 h, cells were stained with ORO, pictured using an inverted phase contrast microscope, and quantified by ORO staining areas. Scale bar: 100 μm. b Statistical analysis of ORO staining. For cellular TC and TG test, cells were digested by trypsin, washed with PBS and collected using centrifuge. The following steps were performed according to the manufacturer’s protocols. All experiments were repeated at least three biological times. Bars = means ± SD; n = 3–10; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

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