Fig. 2

Tamoxifen inhibited lipid accumulation in mice fed with normal diet. a Dosing scheme of tamoxifen on male and female C57BL/6 mice fed with normal diets. Dose of tamoxifen: 100 mg/kg. b Since initiating tamoxifen administration, mice were pair-fed and body weight was recorded weekly until mice were sacrificed. c Food consumption weight per cage was recorded when mice were administrated with tamoxifen. d Since initiating tamoxifen administration, mice were free-fed and body weight was recorded every other day for 4 weeks. e Food consumption weight per cage was recorded weekly when free-fed mice were administrated with tamoxifen. f Serum TC, HDL-C and LDL-C analysis of male and female mice fed with normal diets and administrated with vehicle/tamoxifen for 1, 2, 4, 8 weeks, respectively. g GTT test was performed on male and female mice administrated with tamoxifen or vehicle for 8 weeks and area under curve (AUC) was calculated and compared. h ITT test was performed on male and female mice administrated with tamoxifen or vehicle for 8 weeks and area under curve (AUC) was calculated and compared. i Frozen liver sections from male and female mice administrated with tamoxifen or vehicle for 1 and 4 weeks was performed ORO staining and ORO staining area was quantitatively compared. Scale bar:100 μm. j Frozen liver sections from male and female mice administrated with tamoxifen or vehicle for 8 weeks were performed ORO staining and ORO staining area was quantitatively compared. Scale bar:100 μm. Bars = means ± SD; n = 3– 5; ns, no significance; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001