Fig. 1 | Signal Transduction and Targeted Therapy

Fig. 1

From: RPS15 interacted with IGF2BP1 to promote esophageal squamous cell carcinoma development via recognizing m6A modification

Fig. 1The alternative text for this image may have been generated using AI.

High RPS15 expression correlates with malignant phenotype and poor prognosis in ESCC. a Schematic created with BioRender.com demonstrating the screening strategy for pro-metastatic ribosome proteins (RPs). b, c qRT-PCR analysis of fold changes in gene expression of 89 RPs between metastatic and non-metastatic of KYSE30 cells (b) and KYSE450 cells (c). Data were presented as mean ± SD; n = 3. Two-tailed t-tests. d Venn diagram showing the RPs upregulated in metastasis of KYSE30 and KYSE450 cells. e qRT-PCR analysis of fold changes of four RPs expression in highly metastatic ESCC cell lines 30-D-4 and K450LM2. Data were presented as mean ± SD; n = 3. Two-tailed t-tests. f Representative images (left) and the statistical analysis (right) of TMA-based IHC showing higher RPS15 expression in tumor tissue (upper panel). The expression of RPS15 is correlated with lymph node metastasis (lower panel). Scale bars, 50 μm. The data were analyzed using paired two-tailed t-test and Fisher exact test and are presented as mean ± SD. *P < 0.05 and ***P < 0.001. g Kaplan–Meier survival curve showing that high expression of RPS15 correlated with lower disease-free survival in patients with ESCC (P < 0.05). h Multivariate Cox regression analysis of factors associated with survival of patients with ESCC

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