Fig. 4

kshv-miR-K12-1-5p targeted IFN signaling pathway. a Schematic diagram of Ago2 RNA immunoprecipitation and sequencing (Ago2- RIP seq). b Volcano plot of Ago2-RIP seq profile. The upregulated and downregulated genes were indicated by red and blue color codes, respectively, following a filtering criterion as fold change>2 and p < 0.05. c Gene ontology analyses of the upregulated 67 genes. d Heat map of the upregulated genes in type I IFN signaling pathway. e Venn diagram showing the overlap number of candidate target genes of kshv-miR-K12-1-5p identified by Ago2-RIP seq, type I IFN signaling pathway and RNAhybrid. f The RNA levels pulled down by anti-Ago2 antibody in hiPSC-derived CM (n = 3 each). g The RNA levels pulled down by biotin-labeled kshv-miR-K12-1-5p in AC16 cells (n = 4 each). h The expression of candidate target genes detected by Western blot in AC16 cells with kshv-miR-K12-1-5p transfection followed by IFNβ treatment (n = 5 each). *p < 0.05