Fig. 5

Albumosomes regulate CPT2 sorting to mitochondria to maintain mitochondrial homeostasis. a–d Confocal microscopy of HEK293T transfected with GFP-Albumin and BFP-CPT2 (a), GFP-Albumin and BFP-Vector (b), GFP-Vector and BFP-CPT2 (c), GFP-Vector and BFP-Vector (d). Mito-tracker was used for mitochondria staining. Representative images were shown. The intensities along the yellow arrows were measured. Scale bars: 5 μm. e HEK293T mitochondria isolation assay. Mitochondria was isolated from HEK293T transfected with GFP-Albumin and BFP-CPT2 or GFP-Vector and BFP-CPT2. Relative intensity was measured from Western blot results (n = 3). f IF of albumin and mitochondrial folded CPT2 of HepG2-WT and HepG2-AKO. Mito-tracker was used for mitochondria staining. Representative images were shown. Intensities of albumin and folded CPT2 were measured. Scale bars: 5 μm. g Seahorse mitochondrial respiration assay of HepG2-WT and HepG2-AKO overexpressed with GFP-Vector or GFP-CPT2, respectively. Basal respiration, maximal respiration, spare capacity, ATP production, and proton leak were analyzed. Data are analyzed by unpaired two-tailed Student’s t test and represented as mean ± SEM. ns. represents no significance. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001