Fig. 1

TRIM28 stabilizes PD-L1 in G.C. cells. a Genome-wide CRISPR screen identified genes that possibly regulate PD-L1 expression in N87 gastric cancer cells. b MAGeCK-VISPR algorithm was used to assess the sgRNA abundance in PD-L1 positive and negative cells by calculating the beta score for each gene. c Flow-cytometric analysis of the cell surface PD-L1 expression in AGS cells stably transduced with lentiviruses containing the empty vectors, sh-TRIM28C, or sh-TRIM28D. d SGC-7901 and MGC-803 cells were transfected with TRIM28 encoding constructs, and western blotting was performed to detect TRIM28 and PD-L1 protein levels. e Western blot analysis of TRIM28 and PD-L1 protein levels in AGS and BGC-823 cells stably transduced with lentiviruses containing the empty vectors, sh-TRIM28C, or sh-TRIM28D. f AGS cells were transduced with lentiviruses containing the empty vectors or sh-TRIM28C and incubated with CHX for 0, 2, 4, and 8 h. Following this, the half-life of PD-L1 protein in different groups was evaluated. Bar = means ± SD; n = 3; ns, no significance; *P < 0.05; **P < 0.01; ***P < 0.001