Fig. 3 | Signal Transduction and Targeted Therapy

Fig. 3

From: TRIM28 promotes the escape of gastric cancer cells from immune surveillance by increasing PD-L1 abundance

Fig. 3

TRIM28 protects PD-L1 from proteasome-mediated degradation and promotes SUMOylation of PD-L1. a Western blot analysis of TRIM28 and PD-L1 protein levels in AGS cells stably transduced with lentiviruses containing the empty vectors, sh-TRIM28C, or sh-TRIM28D following treatment with DMSO or MG132 (10 μM). b Co-IP analysis of ubiquitinated PD-L1 in MGC-803 and SGC-7901 cells co-transfected with constructs encoding Myc-PD-L1, Flag-TRIM28, and His-ubi. c Co-IP analysis of ubiquitinated PD-L1 in MGC-803 cells transfected with the indicated constructs. d Western blot analysis of PD-L1 protein levels in SGC-7901 or MGC-803 cells stimulated with different concentrations of 2-D08 for 24 h. e, f SUMOylation assays in the lysates from MGC-803 cells transfected with indicated constructs

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