Fig. 5 | Signal Transduction and Targeted Therapy

Fig. 5

From: TRIM28 promotes the escape of gastric cancer cells from immune surveillance by increasing PD-L1 abundance

Fig. 5

TRIM28 interacts with and activates TBK1 by promoting its K63-linked ubiquitination. a, b Correlation between TRIM28 expression and the expression of PD-L1, TBK1, and IRF1 analyzed using the SRA-PRJEB25780 dataset. c, d Co-IP analysis for evaluating the interaction between TRIM28 and TBK1 in G.C. cells. e Confocal microscopy analysis showing colocalization of TRIM28 (Red) and TBK1 (Green) in SGC-7901 cells. f Western blot analysis of the cytoplasmic and nuclear fractions derived from G.C. cells. g Co-IP analysis of the interaction between TBK1 and TRIM28 full-length or TRIM28 truncated mutants in G.C. cells. h Co-IP analysis of ubiquitinated TBK1 in MGC-803 and SGC-7901 cells transfected with indicated constructs. i Co-IP analysis of ubiquitinated TBK1 in SGC-7901 and MGC-803 cells transfected with indicated constructs

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