Fig. 6: TRIM28-mediated PD-L1 upregulation promotes G.C. tumor growth and inhibits antitumor immunity. | Signal Transduction and Targeted Therapy

Fig. 6: TRIM28-mediated PD-L1 upregulation promotes G.C. tumor growth and inhibits antitumor immunity.

From: TRIM28 promotes the escape of gastric cancer cells from immune surveillance by increasing PD-L1 abundance

Fig. 6

TRIM28-mediated PD-L1 upregulation promotes G.C. tumor growth and inhibits antitumor immunity. a, b Assays evaluating T cell-mediated tumor cell death. c WT and TRIM28 overexpressing MFC cells were laterally injected into the abdomen of 615 mice treated with either anti-PD-L1 antibodies or IgG isotype control and randomly assigned to different groups as indicated. Representative images of the 615 mouse tumors. d, e The volume and weight of the formed tumors. f Immunohistochemistry analysis showing TRIM28 expression, and the number of infiltrated CD8+ T cells in tumors derived from the WT or TRIM28 overexpressing MFC cells. Scale bar, 50 μm. g Analysis of TRIM28 and PD-L1 mRNA expression in G.C. patients using ACRG and TCGA databases. h Correlation between TRIM28 and PD-L1 mRNA levels in G.C. tissues from our cohort. i, Representative immunohistochemical staining of G.C. tumors showing TRIM28 and PD-L1 expression. j Correlation between TRIM28 and PD-L1 protein levels in G.C. tissues from our cohort. k Kaplan-Meier survival analysis based on TRIM28 expression in G.C. patients from our cohort. l Kaplan-Meier survival analysis based on TRIM28 expression in G.C. patients from the GEO database. m TRIM28 expressing MFC cells were laterally injected into the abdomen of 615 mice treated with or without the TBK1 inhibitor amlexanox. n, o The volume and weight of the formed tumors in different groups. p Immunohistochemistry analysis of PD-L1 expression and the number of infiltrated CD8+ T cells in tumors from different groups. Scale bar, 50 μm. q Mice carrying tumors derived from MFC cells were treated with either the TBK1 inhibitor amlexanox or anti-CTLA4 antibodies individually or a combination of both. r, s The volume and weight of the formed tumors in different groups. t Immunohistochemistry analysis of PD-L1 expression and the number of infiltrated CD8+ T cells in tumors from different groups. Scale bar, 50 μm. Bar = means ± SD; n = 3; ns, no significance; *P < 0.05; **P < 0.01; ***P < 0.001

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