Fig. 5
From: VEGF-B prevents excessive angiogenesis by inhibiting FGF2/FGFR1 pathway
VEGF-B inhibits FGF2-induced angiogenesis. a, b Representative images (a) and corresponding quantification (b) of in vivo Matrigel assay showing that VEGF-B inhibits FGF2-induced angiogenesis. n = 8 for FGF2 and FGF2 + VEGF-B, n = 6 for BSA, VEGF-B, FGF2 + PlGF and PlGF. p values are from two-way ANOVA followed by LSD test using the logarithmically transformed data. c, d Representative images (c) of cell migration assays using HRECs and corresponding quantifications of migrated cells (d, n = 12 per group). e–g Representative images (e) of HREC spheroid sprouting assays and corresponding quantifications of the number of sprouts/spheroid (f) and sprout length (g). n = 6, 9, and 10 per group for BSA, VEGF-B (100 ng/ml) and FGF2 (50 ng/ml); n = 8 per group for FGF2 (50 ng/ml) + VEGF-B (100 ng/ml), FGF2 (50 ng/ml) + PlGF (100 ng/ml) and PlGF (100 ng/ml). The experiment was repeated three times. For (d), (f), and (g), adjusted p values are from two-way ANOVA followed by Sidak post hoc analysis (number of comparisons, 9). Scale bars: 50 µm for (a) and (c), 100 µm for (e). A.U.: arbitrary unit. All data are mean ± s.e.m. The experiments were repeated three times
