Fig. 7

RNA-seq analysis of tumor tissues. a Heat map illustrating the differentially expressed genes between the tumor group and the control tissue group (log2FC > 1; Padj < 0.01). b KEGG gene set enrichment analysis based on RNA-Seq data by comparing tumor groups with control tissue groups to identify alterations in tumor-associated signaling pathways (Padj < 0.05). c Heat map showcasing the screening of the tumor-related gene list provided by the COSMIC Cancer Gene Census. The classification is based on the “Role in Cancer” information provided by the COSMIC database. d Heat map displaying the expression of genes related to breast cancer. e Validation of the expression levels of tumor-associated genes using RT-qPCR. Statistical analysis of the data was conducted using an unpaired two-tailed t-test to compare the two groups. A significance level of P < 0.05 was considered statistically significant