Fig. 2

Metabolic profiles across various brain regions. a Three-dimensional diagram of PCA of brain metabolome from mice fed ad libitum or subjected to STF. R2X = 0.614, Q2 = 0.420 (GC-MS data). b Three-dimensional diagram of PCA of brain metabolome from mice fed ad libitum. R2X = 0.869, Q2 = 0.599 (GC-MS data). c Heat map of the differentially expressed metabolites (detected by GC-MS) among seven brain regions in mice fed ad libitum. d Pathway analysis of the highly expressed and lowly expressed metabolites in different brain regions. Pathway enrichment analysis was performed utilizing the Pathway Analysis module from online MetaboAnalyst 5.0 (https://www.metaboanalyst.ca/)