Fig. 4
Visualization and pathway analysis of the differentially expressed lipids and metabolites that responded to fasting in the brain. a Change of total abundance of each lipid class after STF in seven brain regions. *0.01 < p < 0.05, **0.001 < p < 0.01, ***p < 0.001. CAR, acylcarnitine; Cer, ceramide; DG, diacylglycerol; FA, fatty acid; PC, phosphatidylcholine; MG, monoacylglycerol; NAE, N-acylethanolamine; PG, phosphatidylglycerol; PS, phosphatidylserine; CL, cardiolipin; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; SM, sphingomyelin; ST, sterol lipids; TG, triacylglycerol. b Summary of the categories of differential metabolites detected by GC-MS in seven brain regions. c Heat map of DEMs (GC-MS data) in OB after STF. d Percentage of DEMs (GC-MS data) in different metabolic pathways in OB. e Top-25 metabolic pathways (GC-MS data) that were associated with STF in OB. The rich factor indicates the ratio of the number of DEMs in the indicated metabolic pathway to the number of all metabolites annotated to this pathway, the higher the value, the higher the enrichment degree. The “Number” in circle size represents the number of DEMs that fall into the indicated pathway. Pathway enrichment analysis was performed utilizing the Pathway Analysis module from online MetaboAnalyst 5.0 (https://www.metaboanalyst.ca/)
