Fig. 8 | Signal Transduction and Targeted Therapy

Fig. 8

From: NPRC deletion mitigated atherosclerosis by inhibiting oxidative stress, inflammation and apoptosis in ApoE knockout mice

Fig. 8The alt text for this image may have been generated using AI.

Overexpression of NPRC increased inflammation and apoptosis and aggravated migration and phagocytosis of macrophages. a Representative Western blot images of ICAM1, VCAM1, TNFα, MCP1, and IL-6 expression in oxLDL-stimulated oe-NC and oe-NPRC HAECs. b Quantification of ICAM1, VCAM1, TNFα, MCP1, and IL-6 expression in oxLDL-stimulated oe-NC and oe-NPRC HAECs (n = 5 per group). c Representative images of Raw 264.7 migration in oxLDL-stimulated oe-NC and oe-NPRC HAECs (scale bar = 100 μm). d Quantification of crystal violet positive cells in Raw 264.7 stimulated by medium from si-NC and si-NPRC HAECs (n = 5 per group). e Representative images of phagocytosis of Raw 264.7 from si-NC and oe-NPRC HAECs after oxLDL stimulation (scale bar = 100 μm). f Quantification of relative positive staining area of Oil Red O in (e) (n = 5 per group). g Representative images of TUNEL assay in oxLDL-stimulated oe-NC and oe-NPRC HAECs (scale bar = 100 μm). h Quantification of TUNEL-positive cells in oxLDL-stimulated oe-NC and si-NPRC HAECs (n = 5 per group). i Representative Western blot images of p-AKT1, AKT1, Cleaved-caspase 3, Caspase 3, Cleaved-caspase 7, and Caspase 7 expression in oxLDL-stimulated oe-NC and oe-NPRC HAECs. j Quantification of p-AKT1/AKT1, Cleaved-caspase 3/Caspase 3 and Cleaved-caspase 7/Caspase 7 expression in oxLDL-stimulated oe-NC and oe-NPRC HAECs (n = 5 per group). Normal distributions were tested by Shapiro–Wilk method. Unpaired two-tailed Student’s t tests were applied in (d), (f), (h). Two-way ANOVA was used in (b) and (j)

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