Fig. 9 | Signal Transduction and Targeted Therapy

Fig. 9

From: NPRC deletion mitigated atherosclerosis by inhibiting oxidative stress, inflammation and apoptosis in ApoE knockout mice

Fig. 9The alt text for this image may have been generated using AI.

Activation of PKA pathway upregulated eNOS and p-AKT1, and inhibited p-p65 expression. a Representative Western blot images of p-Iκκα/β, Iκκα, Iκκβ, p-P65, and P65 expression in oxLDL-stimulated oe-NC and oe-NPRC HAECs. b Quantification of p-Iκκα/β, Iκκα, Iκκβ, p-P65, and P65 expression in oxLDL-stimulated oe-NC and oe-NPRC HAECs (n = 5 per group). c Representative immunofluorescence images of p-P65 in oe-NC and oe-NPRC HAECs stimulated by oxLDL (scale bar = 50 μm). d Quantification of p-P65 immunofluorescent intensity in nuclei of oe-NC and oe-NPRC HAECs stimulated by oxLDL (n = 5 per group). e Representative Western blot images of p-PKA-substrate expression in HAECs treated by forskolin and H89. f Quantification of p-PKA-substrate expression in HAECs treated by forskolin and H89 (n = 5 per group). g Representative Western blot images of eNOS expression in HAECs treated by forskolin and H89. h Quantification of eNOS expression in HAECs treated by forskolin and H89 (n = 5 per group). i Representative Western blot images of p-P65, P65, p-AKT1, and AKT1 expression in HAECs treated by forskolin and H89. j Quantification of p-P65/P65 expression in HAECs treated by forskolin and H89 (n = 5 per group). k Representative images of TUNEL assay in HAECs pretreated by forskolin and H89, and then stimulated by oxLDL (scale bar = 100 μm). l Quantification of TUNEL-positive cells in HAECs pretreated by forskolin and H89, and then stimulated by oxLDL (n = 5 per group). Normal distributions were tested by Shapiro–Wilk method. Two-way ANOVA was applied in (b). Unpaired two-tailed Student’s t tests were applied in (d). One-way ANOVAs were used in (f), (h), (j), and (l)

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