Fig. 2

CFP1 knockdown inhibited malignant behaviors of LUAD in vitro and vivo. a, b The cell proliferation assay using CCK8 for CFP1-knockdown and control cells, including A549 and H1975 cell lines. c, d The representative colony formation images and quantitative comparison of colony numbers for CFP1-knockdown and control cells, including H1975 and A549 cell lines. (n = 3; mean ± SD). e, f The representative migration and invasion images and quantitative comparison of cell numbers for CFP1-knockdown and control cells. (n = 3; mean ± SD). g Comparison of subcutaneous tumors in two mice groups. h The growth curve of mice subcutaneous tumors in (g). i Box plot of tumor weights in the two mice groups. j, k The represent image and statistical bar plot for Ki67 staining of tumor tissues in (g). Scale bars:50 μm. l, m The represent image and statistical bar plot for Tunel staining to detect the apoptosis in tumor tissues. Scale bars:50 μm. n Comparison of mice lung metastatic lesions and the microscopic images of hematoxylin-eosin staining sections of lung tissues. o The statistical bar plots for the number of metastatic lesions. (for g–o, n = 5; mean ± SD) *P < 0.05; **P < 0.01; ***P < 0.001