Fig. 6

CFP1-mediated H3K4me3 deposition at regulated promoters selectively affected tumor-related gene transcription, which mainly lead to abnormal activation of the Wnt and TGF-β pathways. a The Venn plot for overlapping genes with increased/decreased enrichment of H3K4me3 peaks on promoters and increased/decreased transcription levels respectively following CFP1 knockdown in LUAD cells. b The rank of genes with the most transcription and H3K4me3 modification changes. c The transcriptional level of the top 20 genes with upregulated transcription level and H3K4me3 enrichment in CFP1-knockdown cells compared with control cells by q-PCR. d The transcriptional level of the top 10 genes with downregulated transcription level and H3K4me3 enrichment in CFP1-knockdown cells compared with control cells by q-PCR. e The protein level of molecules functioning in the WNT signaling pathway. f The transcription level of WNT1 inducible signaling pathway protein 1 (WISP1) and WNT-related key molecule c-MYC. g, h The transcription and protein level of molecules functioning in the TGF-β signaling pathway. (for c, d, f, and h, n = 3; mean ± SD). i The overexpression efficiency of CFP1 in H1975 cell. j, k The colony formation assay showed the different growth inhibition efficiencies of SB431542 and IWR-1 on control or CFP1-overexpression cells. l–n The cell apoptosis rates were measured by Annexin-V/PI staining after 24 h of inhibitor treatment (SB431542 50 μM, IWR-1 50 μM). (for j–n, n = 3; mean ± SD). o CCK8 assay measured cell proliferation rate at different time points after inhibitor treatment. Six replicates were set for each group, and the statistical difference of OD value between groups was analyzed by Student’s t-test. (n = 3; mean ± SD). p The protein expression level of molecules functioning in TGF-β signaling pathways in H1975 cells upon CFP1 overexpression along with the two pathway inhibitors treatment. q The protein expression level of molecules functioning in WNT signaling pathways in H1975 cells upon CFP1 overexpression along with the two pathway inhibitors treatment. *P < 0.05; **P < 0.01; ***P < 0.001; ns not significant