Fig. 5 | Signal Transduction and Targeted Therapy

Fig. 5

From: Dynamic single-cell mapping unveils Epstein‒Barr virus-imprinted T-cell exhaustion and on-treatment response

Fig. 5

Enrichment of dysfunctional LAG-3+CD8+ T cells in EBV ( +) GC. a Heatmap showing the expression of immune checkpoints in T-cell subpopulations of EBV positive patients divided by treatment stages. Center number indicates the average expression of immune checkpoints. b mIHC staining in representative EBV (+) and EBV (−) GC samples for the following markers: CD8, GZMK, LAG-3 and DAPI. A dashed box represents the 4.5× enlarged area shown in the right panels with separate channels. White arrows point to cells positive for LAG-3, GZMK and CD8. Scale bar, 25 μm. c mIHC staining in representative EBV (+) and EBV (−) ICC samples for the following markers: CD8, GZMK, LAG-3 and DAPI. A dashed box represents the 4.5× enlarged area shown in the right panels with separate channels. White arrows point to cells positive for LAG-3, GZMK and CD8. Scale bar, 25 μm. d Scatterplot showing indicated gene expression of T-cell subpopulations ordered across pseudotime. The red line represents the variation of gene expression estimated by a generalized linear model. e Boxplot showing the LAG-3 expression in EBV (+) and EBV (−) GC samples (TCGA, ACRG and Yonsei cohorts). The horizontal line shows the median, the box comprises the interquartile range and the whiskers extend to the 5th and 95th percentiles. P values were calculated by a two-sided Wilcoxon test

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