Fig. 1

JOSD2 is overexpressed and associated with poor prognosis in NSCLC patients. a Schematic illustration of global survival analysis. TCGA analysis of overall survival (OS) in NSCLC patients separated by the expression of 97 DUBs individually. b Plot illustrating hazard ratio compared with P value (−log₂) between the expression of 97 DUBs and OS in NSCLC patients. c Cell survival assays in NCI-H1299, PC-9 and NCI-H358 cells transfected with scramble control or indicated DUBs shRNA. The cells were infected with lentiviruses packaged scramble control or indicated DUBs shRNA for 96 h, then these cells were seeded 4000 cells per well in 96-well plates and cultured for 48 h, followed by SRB staining. d Immunohistochemistry (IHC) staining of JOSD2 in a lung cancer tissue array consisting of LUADs (n = 80) (left). Analysis of JOSD2 IHC staining in correlation to TNM stages (right). The evaluation of the IHC staining was performed by pathologist who is blind from the stage information of these patients, IHC score was evaluated by multiplication of positive staining proportions (1 score, <25%; 2 score, 25–50%; 3 score, 50–75%; 4 score, 75–100%) and intensity of protein expressions (1 score, weak staining; 2 score, moderate staining; 3 score, high staining). e Cell proliferation assay and f Colony formation assay in NCI-H1299, PC-9, NCI-H2347, NCI-H2228, NCI-H292 and NCI-H358 cells transfected with scramble control or JOSD2 shRNA (#1, #2). The indicated cell lines were infected with lentiviruses for 96 h, then these cells were seeded 1000 cells per well in 96-well plates or 6-well plates, followed by SRB staining (means ± SD, n = 3). g Images of scramble control and shJOSD2-expressing tumors of NCI-H358 xenografts (at the endpoint). h Tumor volume of scramble control and shJOSD2-expressing tumors of NCI-H358 xenografts, the tail of mice means the tumor is not growing. i Tumor weight of each mice in indicated groups (at the endpoint) of NCI-H358 xenografts (means ± SEM, n = 10). j Images of scramble control and shJOSD2-expressing tumors of NCI-H1299 xenografts (at the endpoint). k Tumor weight of each mice in indicated groups (at the endpoint) of NCI-H1299 xenografts (means ± SEM, n = 8). *P < 0.05; **P < 0.01; ***P < 0.001