Fig. 8
HY041004 efficiently suppresses NSCLC growth in vitro/vivo. a Proliferation assay and b Colony formation assay in NCI-H292, NCI-H358, NCI-H1299 and PC-9 cells. The indicated cell lines were treated with indicated compounds at a series of concentrations for 72 h, followed by cell proliferation assay and colony formation assay. Black dashed line indicates 50% of cell survival. (means ± SD, n = 3). c, d The in vivo effect of HY041004 on NCI-H1299 xenografted tumors (orally, twice daily, 50 mg/kg and 100 mg/kg) for indicated days. c Relative tumor volume of indicated groups was measured every 2 days (mean ± SEM. n = 6/group). d Tumor weight of each mice in indicated groups (at the endpoint). e Schematic illustration of pharmacological inhibitory mechanism of JOSD2 inhibitors. *P < 0.05; ***P < 0.001
