Fig. 2

Knockout of TEAD1 in cardiac fibroblasts attenuates TAC-induced cardiac remodeling. a Schematic for echocardiography and sample collection from 4 groups: TEAD1^fl/fl and TEAD1^fl/flcol1a2⁺ at 4 weeks after sham or TAC. b Left ventricular EF assessed by echocardiography in TEAD1^fl/fl and TEAD1^fl/flcol1a2⁺ mice after 4 weeks sham or TAC (n = 4–10 per group). c Heart sections from TEAD1^fl/fl and TEAD1^fl/flcol1a2⁺ mice after sham or TAC surgery were stained with picrosirius red to visualize collagen deposition (n = 4–10 mice per group; scale bar=50 μm). d. Heart sections from TEAD1^fl/fl and TEAD1^fl/flcol1a2⁺ mice after sham or TAC surgery were stained with WGA to demarcate the cell boundaries (n = 4-10 mice per group; scale bar=20 μm). e Heart sections from TEAD1^fl/fl and TEAD1^fl/flcol1a2+ mice after sham or TAC surgery were stained with hematoxylin and eosin to show whole-heart gross images (n = 4–10 mice per group; scale bar=5 mm). f Representative immunofluorescence images of Collagen I and Collagen III staining in the hearts from TEAD1^fl/fl and TEAD1^fl/flcol1a2⁺ mice after sham or TAC surgery (n = 4 per group; scale bar=20 μm). g Western blot and quantification of α‑SMA and Galectin-3 protein levels in the heart homogenates extracted from TEAD1^fl/fl and TEAD1^fl/flcol1a2⁺ mice after sham or TAC surgery (n = 4 per group). h qRT-PCR analyses of the mRNA levels of ANP, BNP and β-MHC in heart samples from TEAD1^fl/fl and TEAD1^fl/flcol1a2⁺ mice after sham or TAC surgery (n = 4 per group). For all statistical plots, the data are presented as mean ± SD. ns. indicates no significance between the 2 indicated groups. b–d and g by two-way ANOVA with Bonferroni multiple comparison test. h by two-way ANOVA with Dunnett’s T3 post hoc analysis. TAM, tamoxifen; EF, ejection fraction; WGA, wheat germ agglutinin; ANP, atrial natriuretic peptide; BNP, brain natriuretic peptide; β-MHC, β-myosin heavy chain