Fig. 5
From: Eosinophils promote CD8+ T cell memory generation to potentiate anti-bacterial immunity
IL-4 secreted by eosinophils inhibits CD8+ T cells apoptosis after L.m. infection. a Heat map showing upregulated cytokine and chemokine genes in eosinophils after L.m. infection for 8 h in vitro (P adjusted <0.01 and log2 fold change >2), as analyzed by RNA-seq. b The percentages of Annexin-V+ CD8+ T cells upon L.m. infection for 8 h in the presence of 50 ng/ml of indicated exogenous recombinant cytokines or chemokines, measured by FACS. c IL-4 protein levels in the supernatant of eosinophils with or without L.m. infection for 8 h determined by ELISA. d, e Representative dot plots of FACS analysis of CD8+ T cells co-cultured with L.m.-stimulated eosinophils supernatant in the presence of anti-IL-4 antibody at different concentrations or isotype control after L.m. infection (d). The percentages of Annexin-V+ and PD-1+ CD8+ T cells were shown in (e). f Statistical analysis of Annexin-V+ and PD-1+ CD8+ T cells co-cultured with Il4–/– or WT eosinophils at a 5:2 ratio after L.m. infection. g Confocal microscopy of immunofluorescence staining for IL-4 (red) and Siglec-F (green) in the spleen sections from WT mice on d0 and d2 post L.m. infection. Scale bar, 40 μm. Right, frequency of Siglec-F+ eosinophils localizing together with IL-4+ cells. Data are mean ± SD of one representative experiment. Similar results were seen in two or three independent experiments. Unpaired Student’s t tests. **p < 0.01, ***p < 0.001, ****p < 0.0001
