Fig. 6
From: Eosinophils promote CD8+ T cell memory generation to potentiate anti-bacterial immunity
IL-4 inhibits the JNK/caspase-3-mediated CD8+ T cell apoptosis in response to L.m. infection. a–c Representative FACS image and statistical analysis of TLR4-MD2+ (a), Annexin-V+ (b) and PD-1+ (c) CD8+ T cells with or without LLO treatment for 4–6 h. d–f Representative FACS image and statistical analysis of Annexin-V+ (d), PD-1+ (e) and cytoplasmic p-JNK+ (f) of CD8+ T cells with or without JNK inhibitor IQ-1S treatment after L.m. infection. g–i Representative FACS image and statistical analysis of Annexin-V+ (g), PD-1+ (h) and cytoplasmic p-JNK+ (i) of CD8+ T cells with or without exogenous IL-4 treatment after L.m. infection. j Representative FACS image and quantification of MFI of IL-4R expression in CD8+ T cells with or without treatment of exogenous IL-4. k, l Proportions of Annexin-V+ and PD-1+ CD8+ T cells with or without Z-VAD-FMK (k) and Z-DEVD-FMK (l) treatment after L.m. infection as measured by flow cytometry. m Proportions of cleaved Caspase-3+CD8+ T cells with or without IL-4 treatment after L.m. infection as measured by flow cytometry. n CFSE analysis of proliferative CD8+ T cells after anti-CD3/CD28 stimulation with IL-4 treatment or not. Data are mean ± SD of one representative experiment. Similar results were seen in two or three independent experiments. Unpaired Student’s t tests. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001
