Fig. 1

Cell composition of cardiac myxomas. a The UMAP plot presents all sequenced cells based on cell type from 5 patients. b Violin plots show the expression levels of classic markers across different clusters. c The mIHC image displays Ki67+ and Ki67- myxoma cells. Scale bars, 30 μm. d The scatter plot shows differential genes between MC and other cell types. Genes with corrected p values less than 0.05 in the differential analysis are displayed in the plot. The x-axis represents the difference in gene expression proportions between the two cell groups, while the y-axis represents the fold difference in gene expression means between the two cell groups. e The functional network is formed by marker genes of the MC cluster. Edges in the network represent the semantic similarity of GO annotations between genes. f A lollipop chart shows functional enrichment analysis results of marker genes from MC. g Heatmap shows the correlation of gene expression between various cell types. h Two separate UMAP plots depicting cell types in myxoma and normal heart. The left side represents 5 myxoma samples, annotated in this study. The right side represents 2 normal heart samples, annotated from a published study. i Two separate UMAP plots depicting gene expression of PDE3A and SOX9 in myxoma and normal heart. j The mIHC image displays PDE3A+ cells in myxoma and normal tissues. Scale bars, 20 μm. k The dot plot shows the scaled expression of selected genes for each cluster, colored by the average expression of each gene in each cluster scaled across all clusters. Dot size represents the percentage of cells in each cluster with more than one read of the corresponding gene