Fig. 1
From: One step at a time: new insights into double-stranded ribonucleic acid virus assembly
BTV duality model based on a combination of observations in situ and from purified particles using cryo-electron tomography. Infected cells were either grown on EM-grids to be FIB-milled or used for viral particle purification prior to cryo-electron tomography. The 3D reconstruction of the tomograms led to the characterization of assembly intermediates, helping the authors to propose a duality model where RNAs interact with each other and bind to the viral proteins VP3&VP6 to promote the capsid formation. The star-subcore and the pre-subcore undergo expansion following the remainder of each ssRNA threading into the capsid, leading to the displacement of VP6 by VP1, followed by the VP7 coating of the particle. (The cell and test tube images were taken from SMART (Servier Medical ART) resource pictures and used under the open access creative commons license CC BY 4.0 https://creativecommons.org/licenses/by/4.0/)
