Fig. 2 | Signal Transduction and Targeted Therapy

Fig. 2

From: Isoxazole-based molecules restore NK cell immune surveillance in hepatocarcinogenesis by targeting TM4SF5 and SLAMF7 linkage

Fig. 2

Isoxazoles abrogated HCC development via inhibitions of signal activity depending on TM4SF5 expression. a Scheme for TM4SF5-expressing SNU449T7 cell xenograft models with NOD-SCID male mice (6-week-old, n = 10). b Tumor volumes were measured twice a week with caliper and calculated. Tumor weight was measured right after sacrifice and tumor extraction. The maximal and minimal tumor weight values were eliminated as outliers for animal variances upon drug treatment, resulting in data values at n = 8 for graphs and tumor images. c Liver tissues were processed to immunostaining for the indicated molecules. Representative images were shown. Scale bars, 100 μm. d Subconfluent TM4SF5-negative SNU449Cp (Cp) or TM4SF5-positive SNU449T7 cells were treated with vehicle or isoxazoles (3 μM) for 24 h, prior to harvests and immunoblots. * in the immunoblots indicate non-specific bands. *P < 0.05, unpaired Student’s t tests or Two-way ANOVA. Data are represented as mean ± SEM. Data stands for three independent experiments. See also Fig. S4

Back to article page