Fig. 3
TM4SF5-mediated downregulation of SLAMF7 leads to hepatocarcinoma development. a Scheme for NK cell adoptive transfer models in male NOD-SCID mice (6-week-old, n = 10). b Tumor size was measured twice a week with calipers. Tumor weight was measured after sacrifice and tumor extraction. *P < 0.05, unpaired Student’s t test or Two-way ANOVA. Data are represented as the mean ± SEM. c Tumor tissues were immunostained with Slamf7 antibody. d Analysis of NK92 cell cytotoxicity toward Huh7KO-EV or Huh7KO-TM4SF5 cells. e Immunoblots of SNU761-EV or SNU761-TM4SF5 cells treated with vehicle (0) or ST-5-001 at the indicated concentrations for 24 h. f–i Flow cytometry analysis of perforin (f) and SLAMF7 (g) expression in NK92-EV and NK92-TM4SF5 cells without (f) or with (g) vehicle DMSO or ST-5-002 treatment. MFI of perforin or SLAMF7 were graphed. The representative fluorescent image of TM4SF5 (red) in NK92-TM4SF5 cells was inserted (f). Analyses of NK92-EV and NK92-TM4SF5 cell cytotoxicity toward Huh7KO-EV and Huh7KO-TM4SF5 cells without (h) or with (i) overexpression (O/E) of human SLAMF7 in the hepatocytes. *P < 0.05, **P < 0.01, ***P < 0.001, ***P < 0.0001, ns non-significant, unpaired Student’s t test or Two-way ANOVA. Data are represented as the mean ± SEM. Data represents three isolated experiments
