Table 1 Principles, advantages, and disadvantages of different detection techniques
| Â | MS | HRMS | NMR | SERS | Spatial Metabolomics | Biosensors | Fluorescent probe | Isotope Tracing | Optical/Electrochemical Techniques |
|---|---|---|---|---|---|---|---|---|---|
Sensitivity | High Sensitivity | Higher Sensitivity | Medium Sensitivity | High Sensitivity | High Sensitivity | High Sensitivity | High Sensitivity | High Sensitivity | Exist Differences |
Detection limit | Nanomole | Femtomole | Micromole | Nanomole | Nanomole | Nanomole | Nanomole | Picomole | Nanomole |
Selectivity | Selective or Non-Selective Analysis | Selective or Non-Selective Analysis | Commonly used for Non-Selective Analysis | Selective or Non-Selective Analysis | Selective or Non-Selective Analysis | Selective Analysis | Selective Analysis | Selective Analysis | Selective Analysis |
Destruction | Destructive | Destructive | Non-Destructive | Non-Destructive | Non-Destructive | Non-Destructive | Non-Destructive | Non-Destructive | Non-Destructive |
Reproducibility | Moderate | High | High | Moderate | High | High | High | High | High |
Dynamic monitoring | No | No | No | No | Yes | Yes | Yes | Yes | Yes |
Spatial information | No | No | Yes | Yes | Yes | No | Yes | Yes | No |
Sample preparation | Required | Required | Optional | Optional | demanding | Not Required | Not Required | demanding | Not Required |
Time consumption | Lengthy | Lengthy | Rapid | Rapid | Lengthy | Rapid | Rapid | Rapid | Rapid |
In vivo studies | No | No | No | No | No | Yes | Yes | Yes | Yes |
