Fig. 8 | Signal Transduction and Targeted Therapy

Fig. 8

From: Epstein-Barr virus hijacks histone demethylase machinery to drive epithelial malignancy progression through KDM5B upregulation

Fig. 8The alternative text for this image may have been generated using AI.

KDM5B inhibition reduces the malignancy of EBV-associated epithelial tumors. a Western blot assays detecting the protein levels of H3K4me3 and KDM5B in HONE1-EBV, CNE2-EBV, C666-1 and AGS-EBV cells treated with AS-8351 or vehicle control. GAPDH was used as a loading control. b Colony formation ability of HONE1-EBV, CNE2-EBV and AGS-EBV cells described in (a). The quantification of colony numbers is summarized at the right. c Sphere formation ability of HONE1-EBV, CNE2-EBV and AGS-EBV cells described in (a). d Tumor growth curves of NPC PDX model and AGS-EBV cells treated with AS-8351(25 mg/kg) or vehicle every two days. e Tumor size (upper) and weight (bottom) for the tumors excised from (d). f Western blot assays measuring the protein levels of H3K4me3, PLK2, p110α, AKT, p-AKT (Ser 473), mTOR, and p-mTOR (Ser 2448) in tumors from (e). GAPDH was used as a loading control. g Representative IHC staining images showing the protein levels of H3K4me3, PLK2, and p-mTOR (Ser 2448) in PDX tumors from (e). Statistical analysis of the staining results is presented on the left. h A hypothetical model illustrating how EBV-induced KDM5B expression facilitates the progression of EBV-associated epithelial tumors by activating the PLK2/PI3K/AKT/mTOR cascades. Statistical analysis is performed using Student’s t-test, with data presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. SD, standard deviation. Scale bar, 50 μm

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