Fig. 5

The PD-1⁺CD8⁺CD45RA⁺CCR7⁺ T cells have both regulatory and cytotoxicity T cell functions. a Outline shows the workflow of PD-1⁺CD8⁺CD45RA⁺ CCR7⁺ T cell functional experiments in vitro in the PB of healthy donors after (D5) G-CSF mobilization. The image was created using Biorender (https://biorender.com/). b Representative plots showing sorted PD-1⁺CD8⁺CD45RA⁺CCR7⁺ T cells and PD-1⁻CD8⁺CD45RA⁺CCR7⁺ T cells for functional experiments by FCM. c Representative plots of proliferating T cells cocultured with PD-1⁺CD8⁺CD45RA⁺CCR7⁺ T cells or PD-1⁻CD8⁺CD45RA⁺CCR7⁺ T cells of healthy donors on D5 in different effector-to-target (E:T) ratios by FCM. d Proliferation rate of T cells cocultured with PD-1⁺CD8⁺CD45RA⁺CCR7⁺ T cells or PD-1⁻CD8⁺CD45RA⁺CCR7⁺ T cells of healthy donors on D5 in different effector-to-target (E:T) ratios for 3 days and inhibition rate of PD-1⁺CD8⁺CD45RA⁺CCR7⁺ T cells and PD-1⁻CD8⁺CD45RA⁺CCR7⁺ T cells by FCM (n = 6). e Representative plots of Raji-GFP cells cocultured with PD-1⁺CD8⁺CD45RA⁺CCR7⁺ T cells or PD-1⁻CD8⁺CD45RA⁺CCR7⁺ T cells of healthy donors on D5 in different effector-to-target (E:T) ratios by FCM. f Dead rate of Raji-GFP cell cocultured with PD-1⁺CD8⁺CD45RA⁺CCR7⁺ T cells or PD-1⁻CD8⁺CD45RA⁺CCR7⁺ T cells of healthy donors on D5 in different effector-to-target (E:T) ratios for 4 h and cytotoxicity rate of PD-1⁺CD8⁺CD45RA⁺CCR7⁺ T cells and PD-1⁻CD8⁺CD45RA⁺CCR7⁺ T cells by FCM (n = 6). Data are presented as the mean ± SEM, *P < 0.05, ***P < 0.001, ****P < 0.0001