Fig. 3

DT-109 attenuates vascular calcification in cynomolgus monkeys. a Hematoxylin and eosin (H&E) and calcification staining (Von Kossa) of the aortic arch (scale bar: 100 μm). b Quantification of calcified area in the aortic arch (vehicle, n = 9; DT-109, n = 10). c Extraction of total RNA from monkey common carotid arterial tissue and detection of runx family transcription factor 2 (RUNX2), SRY-box transcription factor 9 (SOX9), cytochrome p450 oxidoreductase (POR) mRNA expression by real-time PCR (n = 5 for each group). Data are presented as mean ± SD. All statistical comparisons between groups were evaluated by Kruskal–Wallis test