Fig. 5

Myeloid CD38 deficiency alleviated hepatocytic hypoxia/reoxygenation injury in a co-culture condition in vitro. a Representative flow cytometry plots of PI staining, along with the quantitative analysis, for hepatocytes from CD38^fl/fl mice co-cultured with BMDMs from CD38^KO mice after H/R injury. MDA contents (b), LDH activities (c), the mRNA expressions of IL-1B (d), TNF-α (d), NLRP3 (e) and IL-18 (e), and the protein expressions and the quantitative analysis (f) of NLRP3, IL-18 and PPARγ were examined in primary hepatocytes from CD38^fl/fl mice co-cultured with BMDMs from CD38^KO mice after H/R injury. Data are shown as means ± SEM, *p < 0.05, **p < 0.01 and ***p < 0.001, n = 3–6 per group