Fig. 7

Myeloid CD38 deficiency promoted macrophage M2 polarization through activating NAD⁺/SIRT1 pathway in vitro. The mRNA expressions of IL1B (a), CD206 (a), and the protein expressions and the quantitative analysis of SIRT1 (b) and SIRT3 (b) were determined by QPCR and Western blot in BMDMs from CD38^fl/fl and CD38^KO mice after LPS stimulation, respectively. c The mRNA expressions of IL1B and IL-6 were determined by QPCR in BMDMs from CD38^fl/fl mice with the stimulation of hepatocytes-derived conditioned media after hypoxia/reoxygenation injury (HCMHR). The protein expressions and the quantitative analysis of SIRT1/SIRT3 (d), the NAD⁺ contents (e) and the infiltrations of the type 2 macrophages (CD206/Il-10, f, g), and type 1 macrophages (CD86/iNOS, f, g) were determined in BMDMs from CD38^KO and CD38^fl/fl mice with HCMHR stimulation. Data are shown as means ± SEM, *p < 0.05, **p < 0.01 and ***p < 0.001, n = 3–6 per group