Fig. 2

Abnormal expression of FUBP3, SLBP, RPL10A, RPS3A, and RPS14 affected SARS-CoV-2 replication. Overexpression of host factors increased the expression levels of gRNA and sgRNA of SARS-CoV-2 in Huh7 cells infected with the GD108 strain (MOI = 0.05, 48 h) (a, b, n = 6, c, n = 3). Knockdown of host factors decreased the expression levels of gRNA and sgRNA of SARS-CoV-2 in Huh7 cells infected with the GD108 strain (MOI = 0.05, 48 h) (d–f, n = 3). Overexpression of host factors increased the expression levels of gRNA and sgRNA of SARS-CoV-2 in Huh7 cells infected with the Omicron strain (MOI = 0.05, 48 h) (g–i, n = 3). Knockdown of host factors decreased the expression levels of gRNA and sgRNA of SARS-CoV-2 in Huh7 cells infected with the Omicron strain (MOI = 0.05, 48 h) (j–l, n = 3). m, n Knockdown of host factors decreased the N protein expression level in Huh7 cells infected with GD108 and Omicron strains using laser confocal microscopy. Scale bars, 25 μm. For all figures, experiments were repeated at least three times with similar results. Data are represented as the mean ± SEM. Multiple comparisons were performed using ANOVA with Dunnett’s test. *P < 0.05, **P < 0.01, and ***P < 0.001