Fig. 4 | Signal Transduction and Targeted Therapy

Fig. 4

From: Phosphoglycerate dehydrogenase stabilizes protein kinase C delta type mRNA to promote hepatocellular carcinoma progression

Fig. 4

PHGDH activates mitophagy through PRKCD and inhibits apoptosis. a Representative transmission electron microscopy snapshots depicting mitophagy in MHCC-97H cells. Cells were infected with AdPHGDH and subsequently transfected with PRKCD shRNA lentiviral vector. The arrows were labeled autophagosomes. Scale bar = 500 nm. b Determination of mitochondrial membrane potential in PHGDH-overexpressing MHCC-97H cells supplemented with or without sotrastaurin (2 μM, 24 h). Scale bar = 50 μm. c Mitochondrial membrane potential staining assay of PHGDH-expressing MHCC-97H cells transfected with PRKCD siRNA. Scale bar = 50 μm. d Western blot of mitophagy and apoptosis makers in PHGDH-expressing MHCC-97H cells transfected with PRKCD shRNA lentiviral vector. e Western blot of mitophagy and apoptosis makers in PHGDH-expressing MHCC-97H cells supplemented with or without sotrastaurin (2 μM, 24 h)

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