Fig. 1
From: Pseudohypoxic stabilization of HIF1α via cyclophilin D suppression promotes melanoma metastasis
CypD expression and melanoma metastasis. a CypD expression and overall survival of melanoma patients with metastasis. DFCI86 and TCGA87 data were downloaded from the cBioPortal database (http://www.cbioportal.org).20,21 Overall survival rates in the high CypD expression group (top 25% of patients) and low CypD expression group (bottom 25% of patients) were analyzed and plotted as Kaplan-Meier curves. b Expression of CypD in non-metastatic (Stage II) and metastatic melanoma patients (Stage III). TCGA88 data were obtained from the cBioPortal database and compared with respect to CypD expression. c Expression of mitochondrial chaperones in metastatic tumors. Datasets from patients with metastatic (n = 41) and non-metastatic (n = 40) skin cancer were obtained from the Gene Expression Omnibus database84 (GSE7553), and mRNA levels of mitochondrial chaperones were compared. The results are presented as dot plots. d Immunohistochemical (IHC) and hematoxylin & eosin(H&E) staining of primary tumor tissues from melanoma patients. H&E and IHC staining using a CypD antibody were performed on non-metastatic (n = 5) and metastatic primary tumors (n = 12) (left). CypD expression was quantified using ImageJ (right). Scale bar, 20 μm. e CypD mRNA expression in melanoma patients. Total RNA was extracted from non-metastatic (n = 3) and metastatic primary tumors (n = 3), followed by RT-qPCR analysis. Relative CypD mRNA levels were compared between the two group. f Lung-infiltrating metastasis of CypD knockout (KO) cells. Wild-type (WT) or CypD KO B16F10 cells (1 × 105 cells) were injected into the tail vein of C57BL/6 mice, and the lungs were harvested and analyzed by H&E staining (left). Tumor area was quantified using ImageJ software to examine the metastatic burden (right, n = 5 per group). The scale bar represents 1 mm. g Lung-infiltrating metastases of CypD-overexpressing (OE) cells. B16F10 cells (5 × 105 cells) stably transfected with pcDNA (Control) or pcDNA-CypD (CypD OE) were injected into the tail vein of C57BL/6 mice (n = 5), and analyzed as in (d). Scale bar, 1 mm. Data are presented as the mean ± SEM. **, p < 0.01; ****, p < 0.0001
