Fig. 6 | Signal Transduction and Targeted Therapy

Fig. 6

From: F-box/LRR-repeat protein 12 reorchestrated microglia to inhibit scarring and achieve adult spinal cord injury repair

Fig. 6The alt text for this image may have been generated using AI.

FBXL12 mediates MYH14 K63 ubiquitination to orchestrate the cytoskeletal reorganization of microglia. a Schematic description of immunoprecipitation‒mass spectrometry (IP‒MS) of microglia treated with 200 ng/mL MBP for 4 days. b, c GO-molecular function and cellular component analyses of whole FBXL12-binding proteins. d KEGG pathway analysis of MBP-treated microglia. e Volcano plot of FBP changes in wild-type microglia and MBP-treated microglia. f Immunoblotting of MYH14 and MYH9 in different microglia. g Immunoblotting of MYH14 in WT and Fbxl12−/− microglia treated with 200 ng/mL MBP for the indicated times. h Immunoprecipitation of FBXL12 with MYH14 in WT and Fbxl12−/− microglia. i Images of different microglia stained with F-actin and antibodies against MYH14. Representative microglia are shown in the middle and below magnified windows. Scale bar as indicated. j Crystal violet staining of WT and Fbxl12WT-OE microglia. k Quantification of migrated microglia in j (one-way ANOVA, mean ± SEM; ***P < 0.001, ****P < 0.0001, n = 3). l Images of WT and Fbxl12WT-OE microglia stained with F-actin, with or without MYH14. m Quantification of the number (left, n = 6) and length (right, n = 16) of filopodium in l (one-way ANOVA, mean ± SEM; ****P < 0.0001)

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