Fig. 5 | Signal Transduction and Targeted Therapy

Fig. 5

From: Ultrasound-enhanced Pt-coordinated polymer immunopotentiators and heterogenic fusion membrane-based multifunctional tumor vaccine nanoplatforms for melanoma treatment

Fig. 5The alternative text for this image may have been generated using AI.

ICD effects and immune responses stimulated by CPIP@EV–CM in vitro. a Schematic illustration of cell uptake, SDT/CDT-induced ICD, and BMDC maturation. Created with BioRender. b Immunofluorescence of B16F10 cells stained with a CRT antibody and c the corresponding quantitative analysis after different therapies. d Quantification of ATP and HMGB1 release in B16F10 cells. e FCM analysis of the expression of CD80 and f CD86 of BMDCs. g The corresponding quantitative analysis of mature BMDCs. h Secretory IL-6 and IL-12 in supernatant BMDCs measured via ELISA. n = 3; ns, not significant, **P < 0.01, and ***P < 0.001. For panels b, c, and d of the ICD markers, the detection time point was 24 h after inoculation. For panels e, f, and g of BMDC markers, the detection time point was 12 h after the incubation of the cell supernatants

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