Fig. 5
High-frequency alternatively spliced genes in SCLCs. a Alternative splicing events (ASEs) in the genes most frequently affected in 45 SCLCs. The tumors are arranged from left to right in the top track. b Alignment of FAK, FAK6, FAK7, and FAK6,7. Only the regions flanking Y397 are shown. c The percent spliced-in (PSI) values of FAK transcripts containing Box 6 and Box 7 in tumor and counterpart normal lung tissues. P value, Student’s t test. d-g Models of the FERM-Kinase region of FAK and its variants. The FERM and kinase domains are colored gray, and the activation (A) loop in the kinase domain is colored green. The variant region is in red for each FAK alternative. The linker between the FERM and kinase domains is in yellow in FAK (d), slate in FAK6 (e), cyan in FAK7 (f), and blue in FAK6,7 (g). The Y397 autophosphorylation site in the linker is labeled. The additional tyrosine residues from the insertion (Y414 in FAK7 and Y420 in FAK6,7) are also labeled. h FAK variants were detected via RT‒PCR in paired tumor–normal samples from 37 patients, including 27 patients whose samples were analyzed via bulk RNA-seq. Sanger sequencing was used to confirm these results, as shown in Supplementary Fig. 10b, c. T tumor tissue, N adjacent normal lung tissue
