Fig. 3
HlaH35A promotes Fl-BMDC maturation via ADAM10. a Predicted accurate model of the interaction of HPFs with mouse ADAM10 (pLDDT = 72.7). The predicted local distance difference test score (pLDDT) was used to evaluate per-residue confidence, with values ≥ 70 indicating reliable backbone modeling. b Representative fluorescence images showing interactions of PA0833 or HPF with ADAM10 on the surface of Fl-BMDCs after 6 h of treatment with PA0833, HPF, or HPF with GI254023X (n = 3 per group). The antigens used were PA0833 or HPF. The arrowheads indicate cells that captured the antigens. c Representative flow cytometry histogram (left) and quantification (right) of MHCII+ cell frequencies gated on CD11c+ cells from Fl-BMDCs after 7.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X (n = 6 per group). The data were pooled from two independent experiments. d Representative flow cytometry histogram (left) and quantification (right) of CD40+ cell frequencies gated on CD11c+ cells from Fl-BMDCs after 7.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X (n = 6 per group). The data were pooled from two independent experiments. e Representative flow cytometry histogram (left) and quantification (right) of CD80+ cell frequencies gated on CD11c+ cells from Fl-BMDCs after 7.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X (n = 6 per group). The data were pooled from two independent experiments. f Representative flow cytometry histogram (left) and quantification (right) of CD86+ cell frequencies gated on CD11c+ cells from Fl-BMDCs after 7.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X (n = 6 per group). The data were pooled from two independent experiments. g Serum levels of TNF-α or IL-6 secreted from Fl-BMDCs after 7.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X (n = 6 per group). The data were pooled from two independent experiments. Each data point indicates a biological replicate in (c–g). The data are presented as the means ± s.e.m.s. Statistical significance was tested via one-way ANOVA followed by Tukey’s multiple comparisons test in (c–g). Fl-BMDCs Flt3L-induced bone marrow dendritic cells, pLDDT predicted local-distance difference test
