Fig. 4
HlaH35A activates Notch signaling via ADAM10 in Fl-BMDCs. a Representative western blot (left) and quantification (right) of N1ICD, N2ICD, Notch3, or Notch4 in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X (n = 3 per group). b Quantification of Hey1, Hes1, or Hes5 mRNA expression by qPCR in Fl-BMDCs after 6 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X (n = 3 per group). c Quantification of Adam10, Notch1, Notch2, Notch3, or Notch4 mRNA expression by qPCR in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X (n = 3 per group). d TPMs of the Notch1, Notch2, Notch3, or Notch4 genes in unstimulated Fl-BMDCs from bulk RNA-seq (n = 3 per group). e Quantification of Notch1, Notch2, Notch3, or Notch4 mRNA expression by qPCR in Fl-BMDCs under homeostatic conditions (n = 3 per group). Each data point indicates a biological replicate in (a) and (d). Each data point indicates a biological replicate derived from the average of three technical replicates in (b, c and e). The data are presented as the means ± s.e.m.s. Statistical significance was tested via one-way ANOVA followed by Tukey’s multiple comparisons test in (a–e)
