Fig. 5

Characteristics and temporal dynamics of T-cell subsets in response to NK cell-based therapy. a UMAP of subclustered T/NK cells, labeled in distinct colors. Cell type annotations are shown. b Dot plot showing the expression levels of marker genes in the indicated T-cell subsets or NK/NKT cells. UMAP plots (c) and boxplots (d) showing the distribution and dynamic alteration of T-cell subtypes following NK cell-based therapy. Box middle lines, median; box limits, upper and lower quartiles; box whiskers, 1.5 × the interquartile range. e Pi and Ti analysis of T-cell subsets. f Violin plots showing the dynamic alterations in the inhibitory score and integrin score of T cells in responders (n = 4). The p value was calculated via the Wilcoxon test. g Violin plots indicating the dynamic alterations in the average expression of significant genes in specific T-cell subsets in responders (n = 4). The p value was calculated via a two-tailed paired Student’s t test. h Comparison of the D50 diversity indices of the TCR Vβ repertoire in the peripheral blood of pre- (n = 3, P24-pre, P26-pre, and P27-pre) or posttreated responders (n = 3, P24-post1, P26-post3, and P27-post1). A low D50 diversity index indicates high clonal expression and low diversity. i Distribution of four clonotype groups in pre- or posttreated responders (defined on the basis of the relative frequency of clonotypes). A p value < 0.05 was considered statistically significant (*p < 0.05, **p < 0.01, ***p < 0.001). UMAP, uniform manifold approximation and projection